Remote connection to Jupyter Notebook

Recently, I analyzed a few single cell RNA-seq datasets and experimented with several new tools from recent publication. While it was fun, most datasets were just too large for my poor laptop to process, and I relied a lot on our server.

I have to admit I am not too good an analyst and am spoiled by the freedom interpreted languages provided — to try and error line by line. However, this freedom would be gone if I have to do run my analysis like Rscript my-analysis.R or python my-analysis.py. Besides, an interactive interface to play and receive feedback immediately would also make learning new tools easier.

So, I decided to use Jupyter Notebook because I am not allowed to run RStudio Server here. Here’s a brief note of how I did it.

First, I connect to the server via SSH, and then run jupyter notebook --no-browser --port 8889 (as this nice tutorial suggested). This would start a Jupyter server, which could be then channelled back to my own machine via SSH. In another terminal session, this time on my own machine, ssh -N -L localhost:8888:localhost:8889 username@server.address would channel whatever from port 8889 of the remote server to localhost:8888.

Open the browser, connect to localhost:8888, and I am ready to go.

Usually I would play with a smaller dataset to form my analysis. Then, I load the real dataset to the notebook and run the whole thing. For this one, I don’t really want to see it run but only am interested in the result, so I would do it withjupyter nbconvert --ExecutePreprocessor.timeout=-1 --to notebook --execute myanalysis.ipynb. It runs Jupyter notebook in command line, and the --ExecutePreprocessor.timeout decides after how long jupyter nbconvert should give up on running a cell. In my case, a cell could run for hours, so I set it to -1, allowing a cell to run forever. This is important because the default is something like 30 minutes.

Similar idea works for Rmarkdown. I draft my analysis with a smaller dataset in my local RStudio, upload the .Rmd file, start R session on the server, and rmarkdown::render("myanalysis.Rmd") would do the trick.

Just remember to use screen or tmux to before running your analysis so it would keep going when you close your SSH connection, and when you are done, both Jupyter notebook and SSH channelling could be turned off with ^C.

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Yen-Chung Chen
PhD Student

Yen is a graduate student interested in developmental biology, neurobiology and bioinformatics.

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